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NASC

The European Arabidopsis Stock Centre

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NASC ID: N484

Name: Accumulation and replication of chloroplasts mutants

ABRC stock number: CS484

Description: arc5 isolated from M2 population of EMS mutagenised Arabidopsis ecotype Ler (Lehle seeds, Arizona, USA). arc6 isolated from K. Feldmann's Wassilewskija (WS) T-DNAmutagenised population (NASC N3115). arc6 crossed into Ler background. Distributed as F4 seed.

Donation Date: 1999-01-06

Donated by:  University of York Joanne Marrison
University of York Rachel Leech

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: ethylmethane sulfonate

Background: Ler(Landsberg_erecta)

Pedigree: arc6 crossed into Ler background.

Generation: F4

Segregation: No

Transformation method: Agrobacterium mediated seed transformation (see lines from K. Feldmann).

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • arc5
  • arc5-1
  • arc6-1
  • arc5
  • arc5
  • arc6

Phenotype

arc5arc6 - Similar to arc6 arc6 - Whole mutant plant phenotype has twisted leaves. Mean chloroplast number per cell = 2 (cf. wild type 120). Mean chloroplast size = 1000µm2 (cf. wild type 50µm2). Proplastid division is also retarded.

References

Robertson, E.J., Rutherford, S.M. & Leech, R.M 1996. Characterization of chloroplast division using the Arabidopsis mutant arc5. Plant physiology 112(1):149-59.PubMed ID: 8819323.